Oil palm production must increase in Ghana to meet the increasing demand for palm oil and avoid costly imports. Although maximum fruit bunch (FB) yields of >20 t ha−1 yr−1 are achievable, average FB yields in Ghana are only 7 t ha−1 yr−1. Despite the pressing need to increase palm oil production and improve yields, knowledge of the underlying causes of poor yields in Ghana is lacking. Closing yield gaps in existing plantings in smallholdings and plantations offers great opportunities to increase oil production without area expansion, thus sparing land for other uses. This study sought to understand the magnitude and underlying causes of yield gaps in plantation and smallholder oil palm production systems in Ghana based on a detailed characterization of management practices and yield measurements over a two-year period.